Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices

Figure 2

Blockade of NR2A-containing NMDA receptors is not associated with increased Tau phosphorylation levels at Ser409 and Ser262 sites. Phosphorylation and protein levels were estimated by Western blotting on cell extracts (40 $\mu$g proteins) obtained from acute hippocampal slices treated with 50 nM NVP for 2 hours. Phosphorylated Tau levels, expressed relative to total Tau (i.e., Tau-5) levels, were measured using antibodies raised against Tau phosphorylated at Ser199, Ser262, and Ser409. The data were expressed as percentage of control values and are means ± SEM of 3 measurements per cell extract obtained from 6 different rats. Since these experiments were independently performed, we determined statistical significance using unpaired $T$-Test. ${}^{*}P<.05\text{,}$${}^{***}P<.0001$, NVP-treated versus control.