Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices

Figure 3

NVP-induced Tau phosphorylation is mediated by calcium and the Cdk5 pathway. (a) Phosphorylated Tau levels at Ser199 were estimated by Western blotting on cell extracts obtained from acute hippocampal slices treated with 50 nM NVP for 2 hours alone or in combination with 10 $\mu$M BAPTA-AM or 10 $\mu$M BAPTA. The data are expressed relative to total Tau (i.e., Tau-5) levels. (b) As in A, except for the GSK-3$\beta$ inhibitor SB216367 (10 $\mu$M), the Cdk5 inhibitor roscovitine (10 $\mu$M), or the calpain inhibitor calpeptine (10 $\mu$M) were employed. The data were expressed as percentage of control values and are means ± SEM of 3 measurements per cell extract obtained from 5 different rats. Statistical analysis was performed by one-way ANOVA followed by Neuman-Keuls’ post hoc test. ${}^{***}P<.001$, drug-treated versus control.

(a)

(b)