Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices
Figure 5
Blockade of NR2A-containing NMDA receptors is associated with increased levels of NR1 and NR2B subunits in biotinylated membranes. Glutamate receptor subunit levels were estimated by Western blotting on biotinylated membranes obtained from acute hippocampal slices treated with 50 nM NVP for 2 hours. Biotinylated NR1, NR2B and GluR1 subunit levels were normalized with respective total protein levels estimated in homogenates of hippocampal slices incubated with or without NVP. The data are means ± SEM of 3 measurements obtained from 4 different rats. Since these experiments were independently performed, we determined statistical significance using unpaired -Test. , NVP-treated versus control.
