The NO-cGMP-PKG Signaling Pathway Coordinately Regulates ERK and ERK-Driven Gene Expression at Pre- and Postsynaptic Sites Following LTP-Inducing Stimulation of Thalamo-Amygdala Synapses
Figure 2
High-frequency stimulation of the MGm/PIN promotes ERK-driven IEG expression in both the LA and MGm/PIN. (a) Placement of stimulation electrode and schematic of the experimental protocol. Rats were given HFS or LFS and sacrificed 2 hours after stimulation. (b) Images of Western blots for Arc/Arg3.1, c-Fos, EGR-1, and GAPDH from both LA (top) and MGm/PIN samples (bottom). (c) Mean (±SEM) percent IEG immunoreactivity from LA punches taken from rats given HFS () or LFS (). (d) Mean (±SEM) percent IEG immunoreactivity from MGm/PIN punches taken from rats given HFS () or LFS (). In each figure, IEG levels have been normalized to GAPDH for each sample, and IEG expression on the ipsilateral side has been expressed as a percentage of that on the contralateral side for each rat. * relative to the ipsilateral side N.S. = not significant.
