The NO-cGMP-PKG Signaling Pathway Coordinately Regulates ERK and ERK-Driven Gene Expression at Pre- and Postsynaptic Sites Following LTP-Inducing Stimulation of Thalamo-Amygdala Synapses
Figure 7
Pharmacological blockade of NOS activation in the LA impairs ERK-driven IEG expression in both LA and MGm/PIN following HFS. (a) Images of Western blots for Arc/Arg3.1, c-Fos, EGR-1 and GAPDH from both LA (top) and MGm/PIN (bottom) samples. (b) Mean (±SEM) percent Arc/Arg3.1, c-Fos and EGR-1 immunoreactivity from LA punches taken from rats given intra-LA infusion of 50% DMSO (vehicle; ) or 1 μg/side 7-Ni (). (c) Mean (±SEM) percent Arc/Arg3.1, c-Fos and EGR-1 immunoreactivity from MGm/PIN punches taken from rats given intra-LA infusion of 50% DMSO (vehicle; ) or 1 μg/side 7-Ni (). In each figure, IEG levels have been normalized to GAPDH for each sample, and IEG expression on the ipsilateral side has been expressed as a percentage of that on the contralateral side for each rat. * relative to the ipsilateral side N.S. = not significant.
