Research Article

Synaptic Remodeling in the Dentate Gyrus, CA3, CA1, Subiculum, and Entorhinal Cortex of Mice: Effects of Deprived Rearing and Voluntary Running

Figure 2

Dark field light microscopy images of the dentate gyrus (DG), CA3, CA1, subiculum (Sub), and entorhinal cortex (EC) with silver-impregnated lysosomal accumulations appearing as bright precipitates (Gallyas technique). In the dentate gyrus, five measuring fields were positioned in the outer (oML), intermediate (mML), and inner molecular layer (iML), as well as in the granule cell (gcl) and subgranular layer of both the suprapyramidal and infrapyramidal blades (not indicated). In CA3, measuring fields were positioned in the stratum radiatum (Sr), stratum lucidum (Sl), stratum pyramidale (Sp), and stratum oriens (So). In CA1, an additional field was set in the stratum lacunosum-moleculare (Slm). One measuring field was positioned in the subiculum and two in the entorhinal cortex in lamina II (EC II) and III (EC III). Scale bar = 200 μm.
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