Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells

Figure 3

Postnatal and adult neurogenic regions express eGFP. (a) The P14 SVZ is replete with eGFP+ cells. Scale bar = 250 microns. (b) The majority of eGFP+ cells surounding the ventral lateral ventricle are in the ependymal layer (arrow). Scale bar = 20 microns. (c) eGFP expression is still robust in the SVZ at P84. Scale bar = 100 microns. (d) Excellent overlap between eGFP fluoresecence and GFP detected with antibodies (P84 SVZ). Scale bar = 50 microns. (e)–(g) Olfactory bulb labeling. eGFP transgene labels newborn neurons in the granule (gr) (f) and periglomerular (pgl) (g) layers.

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