Figure 3: Light microscopic immunohistochemical demonstration of the BrdU-labelled doublets in DG as a result of apparent mitotic division of cells. Postmitotic BrdU immunoperoxidase-labelled cells in different zones of DG on coronal slices (50 μm thick). (a–c) Localization of postmitotic cells near subgranular zone (SGZ). (d–f) Possible migration of postmitotic cells from SGZ (e) to supragranular zone of DG (d). (f) Because of the thickness of brain slices different BrdU-labelled cells are frequently located at various depths in the 50 μm slice, in (f) (inset) the same cells can be seen at different focal planes. Scale bar = 50 μm.