Intracellular Ca²⁺ Stores and Ca²⁺ Influx Are Both Required for BDNF to Rapidly Increase Quantal Vesicular Transmitter Release

Figure 3

BDNF requires Ca²⁺ influx and intracellular Ca²⁺ mobilization to increase mEPSC frequency. (a) Top: representative trace from a CA1 pyramidal cell in the absence of extracellular Ca²⁺ prior to and following BDNF application; left: representative running average plot of mEPSC frequency in the absence of extracellular Ca²⁺; right: mean frequency of mEPSCs before and after BDNF in the absence of extracellular Ca²⁺. (b) Top: representative trace from a CA1 pyramidal cell in the presence of the SERCA pump inhibitor thapsigargin (1 μM) prior to and following BDNF application; left: representative running average plot of mEPSC frequency in the presence of thapsigargin; right: mean frequency of mEPSCs before and after BDNF in the continuous presence of thapsigargin. (c) Top: representative trace from a CA1 pyramidal cell loaded with BAPTA prior to and following BDNF application; left: running average plot of mEPSC frequency in a representative BAPTA-loaded neuron, right: mean frequency of mEPSCs before and after BDNF in BAPTA-loaded neurons.

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