Neural Plasticity / 2012 / Article / Fig 5

Review Article

Multiple Approaches to Investigate the Transport and Activity-Dependent Release of BDNF and Their Application in Neurogenetic Disorders

Figure 5

Neuron expressing BDNF-pHluorin and loaded with the Ca2+ indicator fura-2 AM (image is of 488 nm-excited BDNF-pHluorin). This cell was sensitive to pH (as in Figure 4) and responded to electrical stimulation with Ca2+ transients (using 380 nm excitation, as in Figure 5). Consistent with activity-dependent BDNF release, dendritic BDNF-pHluorin puncta show decreases in intensity (~20% deltaF/F) as a result of BDNF-pHluorin discharge from vesicles following full fusion.
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