Tumor Necrosis Factor Alpha Mediates GABA_A Receptor Trafficking to the Plasma Membrane of Spinal Cord Neurons In Vivo

Figure 3

Quantitative western blotting reveals a nonsignificant trend towards an increase in plasma membrane GABA_AR. (a) Representative examples of Western Blots of membrane-enriched homogenate fractions from an albumin subject and a TNFα subject that were run on the same gel. GABA_AR (red) and plasma membrane protein N-Cadherin (NCad; green) bands were visualized and quantified using the Odyssey IR Imaging System (Li-Cor). (b) Linear intensity quantification of Western Blots of the P2 fraction yields a trend suggesting an increase in GABA_AR : NCad ratio following TNFα injection (𝑃=0.315, 𝑛=10 albumin subjects, 𝑛=8 TNFα subjects). Bars represent group intensity means averaged across 2 Western Blots. Error bars indicate SEM.

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