Regulation of Ca²⁺/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal

Table 1

α-CaMKII immunogold labeling.

		All synapses (≥0 particles)							≥1 particle in respective compartment
		Bouton		Spine		PSD			Bouton	Spine	PSD
Rat	Synapses sampled	Labeled (%) ≥1 particle	Number of particles	Labeled (%) ≥1 particle	Number of particles	Labeled (%) ≥1 particle	Particles/PSD length	PSD length (μm)	Number of particles	Number of particles	Particles/PSD length	PSD length (μm)
Control
1	43	14.0	0.19	20.9	0.60	11.6	1.02	0.184	1.33	2.89	8.77	0.206
2	56	28.6	0.61	46.4	0.89	28.6	2.79	0.156	2.13	1.92	9.76	0.162
3	43	25.6	0.58	58.1	1.74	27.9	1.93	0.196	2.27	3.00	6.90	0.224
4	44	15.9	0.27	40.9	1.18	18.2	2.07	0.170	1.71	2.89	11.37	0.182
5	53	58.5	1.47	84.9	2.66	54.7	6.61	0.170	2.52	3.13	12.07	0.175
	Total 239
Mean ± SEM		28.5 ± 8.0	0.62 ± 0.23	50.2 ± 10.6	1.41 ± 0.36	28.2 ± 7.3	2.88 ± 0.97	0.175 ± 0.007	1.99 ± 0.21	2.77 ± 0.22	9.77 ± 0.92	0.190 ± 0.011
FZP-withdrawn
1	45	11.1	0.20	17.8	0.27	6.7	0.44	0.165	1.80	1.50	6.56	0.159
2	44	27.3	0.52	56.8	0.98	29.5	1.92	0.170	1.92	1.72	6.50	0.178
3	51	29.4	0.90	45.1	1.27	11.8	0.95	0.158	3.07	2.83	8.04	0.152
4	44	22.7	0.36	38.6	0.75	9.1	0.54	0.168	1.60	1.94	5.92	0.169
5	54	16.7	0.17	59.3	1.02	22.2	1.64	0.169	1.00	1.72	7.37	0.172
	Total 238
Mean ± SEM		21.4 ± 3.4	0.43 ± 0.13	43.5 ± 7.5	0.86 ± 0.17	15.9 ± 4.3	1.10 ± 0.29	0.166 ± 0.002	1.88 ± 0.34	1.94 ± 0.23	6.88 ± 0.37	0.166 ± 0.005
𝑃 value		0.44	0.48	0.62	0.20	0.19	0.12	0.25	0.78	0.03 ^*	0.02 ^*	0.08

^∗𝑃<0.05, Student’s t-test.