Regulation of Ca²⁺/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal

Table 2

pCaMKII immunogold labeling.

		All synapses (≥0 particles)							≥1 particle in respective compartment
		Bouton		Spine		PSD			Bouton	Spine	PSD
Rat	Synapses sampled	Labeled (%) ≥1 particle	Number of particles	Labeled (%) ≥1particle	Number of particles	Labeled (%) ≥1 particle	Particles/PSD length	PSD length (μm)	Number of particles	Number of particles	Particles/PSD length	PSD length (μm)
Control
1	43	37.2	1.28	53.5	1.37	30.2	2.60	0.184	3.44	2.57	8.61	0.183
2	56	21.4	0.32	12.5	0.20	8.9	0.98	0.156	1.50	1.57	11.01	0.162
3	43	81.4	3.56	67.4	2.70	34.9	3.94	0.196	4.37	4.00	11.29	0.218
4	44	38.6	0.82	20.5	0.48	11.4	0.66	0.170	2.12	2.33	5.77	0.177
5	53	56.6	1.42	30.2	0.55	28.3	2.75	0.170	2.50	1.81	9.72	0.189
	Total 239
Mean ± SEM		47.0 ± 10.2	1.48 ± 0.55	36.8 ± 10.3	1.06 ± 0.45	22.7 ± 5.3	2.19 ± 0.61	0.175 ± 0.007	2.79 ± 0.51	2.46 ± 0.43	9.28 ± 1.00	0.186 ± 0.009
FZP-withdrawn
1	45	44.4	0.91	33.3	0.73	17.8	1.87	0.165	2.05	2.20	10.51	0.167
2	44	47.7	1.11	13.6	0.20	11.4	0.86	0.170	2.33	1.50	7.59	0.199
3	51	74.5	2.27	54.9	1.08	23.5	2.68	0.158	3.05	1.96	11.37	0.174
4	44	79.5	3.30	63.6	1.86	25.0	1.70	0.168	4.14	2.93	6.80	0.196
5	54	61.1	1.59	44.4	0.91	29.6	3.12	0.169	2.61	2.04	10.52	0.192
	Total 238
Mean ± SEM		61.4 ± 7.0	1.83 ± 0.44	42.0 ± 8.7	0.96 ± 0.27	21.5 ± 1.3	2.05 ± 0.39	0.166 ± 0.002	2.84 ± 0.37	2.13 ± 0.23	9.36 ± 0.91	0.186 ± 0.006
𝑃 value		0.28	0.63	0.71	0.85	0.84	0.85	0.25	0.94	0.52	0.96	0.99