Regulation of Ca²⁺/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal

Table 3

CaMKIIα and pCaMKII immunogold non-PSD synaptic labeling.

Presynaptic

Postsynaptic

Active zone

Membrane

Intracellular

Perisynaptic membrane

Extrasynaptic membrane

Intracellular

Labeled (%) ≥1 particle

Mean Number of particles

Labeled (%) ≥1 particle

Mean Number of particles

Labeled (%) ≥1 particle

Mean Number of particles

Labeled (%) ≥1 particle

Mean Number of particles

Labeled (%) ≥1 particle

Mean Number of particles

Labeled (%) ≥1 particle

Mean Number of particles

CaMKII

CON

4.0 ± 1.3

0.04 ± 0.01

3.0 ± 1.5

0.04 ± 0.02

26.2 ± 7.6

0.54 ± 0.20

0.8 ± 0.5

0.02 ± 0.01

3.9 ± 1.4

0.07 ± 0.04

38.8 ± 9.9

0.86 ± 0.22

FZP

0.8 ± 0.5

0.008 ± 0.005

2.6 ± 1.3

0.03 ± 0.01

19.8 ± 3.0

0.39 ± 0.12

2.6 ± 1.6

0.03 ± 0.02

4.8 ± 2.6

0.06 ± 0.04

32.2 ± 5.7

0.59 ± 0.12

𝑃 value

0.049 ^*

0.047 ^*

0.83

0.88

0.45

0.54

0.37

0.70

0.75

0.94

0.58

0.32

pCaMKII

CON

7.2 ± 2.3

0.09 ± 0.03

4.5 ± 2.5

0.10 ± 0.08

43.7 ± 9.7

1.29 ± 0.46

0.9 ± 0.9

0.01 ± 0.01

2.3 ± 1.1

0.02 ± 0.01

21.9 ± 10.5

0.62 ± 0.34

FZP

7.3 ± 2.2

0.08 ± 0.03

6.7 ± 3.0

0.09 ± 0.04

58.6 ± 7.6

1.67 ± 0.40

3.1 ± 2.2

0.04 ± 0.03

5.1 ± 2.8

0.07 ± 0.05

26.9 ± 7.8

0.49 ± 0.18

𝑃 value

0.98

0.96

0.60

0.87

0.26

0.56

0.40

0.38

0.37

0.36

0.71

0.76

^∗𝑃<0.05, Student’s t-test. Values represent mean ± SEM.