Kalirin, a Key Player in Synapse Formation, Is Implicated in Human Diseases

Figure 2

Kalirin-12, not Kalirin-7, is localized to the growth cone of hippocampal neurons. Primary cultures of hippocampal neurons prepared at embryonic day 20 (E20) were transfected with a vector encoding GFP on the day of culture preparation as described [19]. On day 3 after transfection, cultures were fixed for staining of filamentous actin with Alexa Fluor 633 Phalloidin (Life Technologies) and endogenous Kalirin-7 or Kalirin-12 with isoform-specific rabbit antibodies. Rabbit antibodies were visualized using Cy3 donkey anti-rabbit IgG (The Jackson Laboratory). Images were collected with a Zeiss confocal microscope LSM510.