Neural Plasticity / 2012 / Article / Tab 1

Review Article

Functional Role of Adult Hippocampal Neurogenesis as a Therapeutic Strategy for Mental Disorders

Table 1

Select animal studies investigating the effect of depression on adult hippocampal neurogenesis and behavior.

SpeciesStress paradigmExperimental approachEffects on neurogenesisEffects on behaviorReferences

Physical stress

Sprague-Dawley ratAcute restraint stress:
either 2 or 6hours.
Chronic restraint stress:
6 hours daily for 21 days.
Repeated restraint stress:
6 hours daily for 42 days.
Proliferation: BrdU (1 × 200 mg/kg, ip) at 2 hours pulsing chase
Survival: BrdU (100 mg/kg, ip) daily for 4 days and analysis on 12 days to 3 weeks periods after BrdU injection.
Acute stress: no effect on cell proliferation.
Chronic stress: Decreased cell proliferation, slightly decreased survival rate with no statistical significance.
Repeated stress: decreased survival rate.
Not studied (NS)[11]

Sprague-Dawley rat60 inescapable foot shocksProliferation: BrdU (1 × 100 mg/kg, ip) at 2 hours pulsing chase.
Survival: BrdU (100 mg/kg, ip) on day 9 and analysis 28 days later.
Decreased cell proliferation.
No change on survival rate and differentiation.
NS[12]

ICR mouseChronic restraint stress: 6 hours per day for four weeks.Proliferation: Ki67 immunostaining.
Survival: BrdU (3 × 75 mg/kg, ip) at 6-hour intervals and 24 hours before exposure to chronic restraint stress.
Decreased cell proliferation and survival rate.Impaired context dependent memory.[13]

Wistar ratOlfactory bulbectomy
(OB).
Proliferation: BrdU (1 × 40 mg/kg, ip) at 2 hours pulsing chase.
Survival: BrdU (3 × 200 mg/kg, ip) at 2-hour intervals and analysis 4 weeks after last administration.
Decreased cell proliferation and neuronal differentiation.Impaired context-dependent memory.[14]

Social stress

Sprague-Dawley ratPsychosocial stress by introducing intruder animal.Proliferation: IdU (4 × 57.5 mg/kg, ip) at 12-hour intervals. IdU at 3-day pulsing chase after the first administration.
Short-term survival: BrdU (6 × 50 mg/kg, ip), 12-hour intervals. BrdU at 10-day pulsing chase after first administration.
No change on cell proliferation, but decreased short-term survival rate.NS[15]

Prairie voleSocial isolation: single-housed for 6 weeks.Proliferation: Ki67 immunostaining.
Survival: BrdU (100 mg/kg, ip) daily for fourteen consecutive days and then 6-week pulsing chase after last administration.
Decreased cell proliferation and neuronal maturation.Higher levels of anxiety-like and depression-like behaviors[16]

Wistar ratSocial defeat stress daily for 18 days Proliferation: BrdU (200 mg/kg, ip) at 24-hour pulsing chase.
Survival: BrdU (200 mg/kg, ip) two times per day for 2 days.
Decreased cell proliferation and survival rate. NS[17]