Neural Plasticity / 2012 / Article / Tab 6

Review Article

Functional Role of Adult Hippocampal Neurogenesis as a Therapeutic Strategy for Mental Disorders

Table 6

Select animal studies investigating the effect of exercise on adult hippocampal neurogenesis.

SpeciesType of exerciseExperimental paradigmEffect on neurogenesisEffects on behaviorReferences

C57BL/6 mouseLearners: 2 trials of Morris water maze training per day over 30 days. (with platform).
Swimmers: 2 trials of morris water maze training per day over 30 days (without platform).
Runners: 1 running wheel for 3-4 mice in rat cage.
Proliferation: BrdU (12 × 50 mg/kg, ip) daily for 12 days, then 24-hour pulsing chase.
Survival: BrdU (12 × 50 mg/kg, ip) daily for 12 days, then 40-week pulsing chase.
Learners: decreased cell proliferation and survival of newly generated neurons.
Swimmers: decreased cell proliferation and survival of newly generated neurons
Runners: increased cell proliferation and survival of newly generated neurons.
NS[53]

C57BL/6 mouseVoluntary running.MRI scan at weeks 0, 2, 4, and 6.
Survival: BrdU (7 × 60 mg/kg, ip) during second week of experiment, then 4-weeks pulsing chase.
Exercise:
(i) increased cerebral blood volume specifically at dentate gyrus.
(ii) increased neuronal maturation.
NS[54]

Hes5::GFP mouseVoluntary running.BrdU (50 mg/kg, ip) either once or 3 consecutive times (2-hour interval); then 2 hour or 5-day pulsing chase. Exercise activated QNPs in dentate gyrus.NS[55]

C57/BL6 mouseVoluntary running.Spatial pattern separation.
BrdU (5 × 50 mg/kg, ip) daily for 5 days, then 10-week pulsing chase.
Exercise increased neuronal maturation.Exercise increased spatial pattern separation.[56]

C57/BL6 mouseVoluntary running.Spatial learning:
Morris water maze task day 35–39.
Proliferation: BrdU (7 × 50 mg/kg, ip) daily for 7 days, then 24-hour pulsing chase.
Neuronal morphology analysis:
4-week dpi GFP retrovirus.
Blood vessel size analysis:
Lectin staining
Proliferation: increased proliferation in both young and old mice.
Neuronal morphology analysis: increased dendritic length and branches in young runners.
increased blood vessel size in young runners only.
Exercise enhanced spatial learning in both young and old mice.[57]