Select animal studies investigating the effect of exercise on adult hippocampal neurogenesis.
|Species||Type of exercise||Experimental paradigm||Effect on neurogenesis||Effects on behavior||References|
|C57BL/6 mouse||Learners: 2 trials of Morris water maze training per day over 30 days. (with platform).|
Swimmers: 2 trials of morris water maze training per day over 30 days (without platform).
Runners: 1 running wheel for 3-4 mice in rat cage.
|Proliferation: BrdU (12 × 50 mg/kg, ip) daily for 12 days, then 24-hour pulsing chase.|
Survival: BrdU (12 × 50 mg/kg, ip) daily for 12 days, then 40-week pulsing chase.
|Learners: decreased cell proliferation and survival of newly generated neurons.|
Swimmers: decreased cell proliferation and survival of newly generated neurons
Runners: increased cell proliferation and survival of newly generated neurons.
|C57BL/6 mouse||Voluntary running.||MRI scan at weeks 0, 2, 4, and 6.|
Survival: BrdU (7 × 60 mg/kg, ip) during second week of experiment, then 4-weeks pulsing chase.
(i) increased cerebral blood volume specifically at dentate gyrus.
(ii) increased neuronal maturation.
|Hes5::GFP mouse||Voluntary running.||BrdU (50 mg/kg, ip) either once or 3 consecutive times (2-hour interval); then 2 hour or 5-day pulsing chase. ||Exercise activated QNPs in dentate gyrus.||NS|||
|C57/BL6 mouse||Voluntary running.||Spatial pattern separation.|
BrdU (5 × 50 mg/kg, ip) daily for 5 days, then 10-week pulsing chase.
|Exercise increased neuronal maturation.||Exercise increased spatial pattern separation.|| |
|C57/BL6 mouse||Voluntary running.||Spatial learning:|
Morris water maze task day 35–39.
Proliferation: BrdU (7 × 50 mg/kg, ip) daily for 7 days, then 24-hour pulsing chase.
Neuronal morphology analysis:
4-week dpi GFP retrovirus.
Blood vessel size analysis:
|Proliferation: increased proliferation in both young and old mice.|
Neuronal morphology analysis: increased dendritic length and branches in young runners.
increased blood vessel size in young runners only.
|Exercise enhanced spatial learning in both young and old mice.|| |