Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
Figure 5
Top: confocal microscope images of Phalloidin-Alexa568 from Stratum radiatum of CA1 hippocampal tissue from 4-month-old control and asphyctic rats. A decrease in the punctate staining was observed after 19 minutes of PA (arrows). The assessment of the percentage of the reactive area from Stratum radiatum of CA1 hippocampal Phalloidin-Alexa568 staining in PA rats showed a decrement in the reactivity area staining with phalloidin. *. Bars and error bars represent mean + SEM. DEN: dendrites; CB: cell body. Scale bar: 10 μm. Bottom: electron micrograph of photooxidated area in the Stratum radiatum of CA1 hippocampal tissue of 4-month-old rats. Arrows point out the dendritic spines stained. A decrement in the number of the F-actin-positive spines was observed after 19 min of PA. AT: axon terminal; DEN: dendritic shaft. Scale bar: 1 μm. The graph shows the assessment of the number of spines per field from Stratum radiatum of CA1 hippocampal slices. A significant decrement in the number of positive F-actin spines was observed in the PA group in comparison with the CTL group. *. Bars and error bars represent mean + SEM.
