The Plasma Membrane Ca²⁺-ATPase2 (PMCA2) Is Involved in the Regulation of Purkinje Cell Dendritic Growth in Cerebellar Organotypic Slice Cultures

Figure 1

Staining of anti-calcium pump PMCA2 ATPase and calbindin D-28K in Purkinje cells in organotypic slice cultures: (a–c) untreated control cultures, (d–f) carboxyeosin-treated cultures. (a) Untreated control showing abundant expression of PMCA2 in dendrites and dendritic spines (green). (b) Merged red Purkinje cell soma with higher expression of PMCA2 in dendrites. (c) Abundant expression of PMCA2 in dendritic plasma membrane and dendritic spines. (d) Carboxyeosin-treated Purkinje cell showing abundant expression of PMCA2 in dendrites and dendritic spines (green). (e) Red Purkinje cell soma with higher expression of PMCA2 in dendrites and dendritic spines. (f) Abundant expression of PMCA2 in dendritic plasma membrane and dendritic spines. Scale bar 50 μm (a-b, d-e) and 10 μm (c–f).

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