The Plasma Membrane Ca2+-ATPase2 (PMCA2) Is Involved in the Regulation of Purkinje Cell Dendritic Growth in Cerebellar Organotypic Slice Cultures
Figure 3
(a–d) Anti-calbindin-stained Purkinje cells from different pharmacological treatment. (a) Purkinje cells from untreated control cultures have a well-defined and profuse dendritic arbor. (b) Purkinje cells from slice culture treated with 10 μM DHPG have a dendritic arbor greatly reduced in size with an absence of the distal dendritic branches. (c) Purkinje cells from carboxyeosin-treated cultures have a dendritic arbor smaller compared to untreated control but larger compared to DHPG treated cultures. (d) Cotreatment with 20 μM carboxyeosin provides a partial rescue of the distal dendritic branches of the Purkinje cells. The size of the dendritic arbor is larger compared to DHPG treatment alone. Scale bar 50 μm.
