Microglia: An Active Player in the Regulation of Synaptic Activity

Figure 1

Microglia alter the synaptic density of hippocampal neurons. Hippocampal neurons with or without microglia were stained with PSD95 (green), synapsin I (blue), and phalloidin (red) (a). The smaller boxes show magnified images. Arrows depict PSD95⁺ synapsin 1⁻ puncta. Scale bars: 20 μm (upper panel); 5 μm (lower panel). Quantification of spine numbers (b), PSD95⁺synapsin 1⁺ puncta (c), and PSD95⁺ synapsin 1⁺ puncta in total PSD95⁺ puncta (d) in neurons cultured with or without microglia. Values are presented as mean±SEM and expressed as a percent of the neurons-only control sample (adapted from [28]).

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