Microglia: An Active Player in the Regulation of Synaptic Activity
Figure 2
Microglial deficiency preserves the levels of synaptic adhesion molecules. (a) Hippocampal neurons at 19 DIV were cocultured with microglia for 2 days. (b) The western blot analysis of the levels of N-cadherin, pan-γ-protocadherin, and SynCAM-1 in neurons in the absence or presence of microglia from wild-type (WT) or mice. α-Tubulin was used as a loading control. (c) Quantification was performed using the ImageJ software and normalized against α-tubulin (). * compared to neurons alone.