Figure 3: Immunohistochemistry for GFAP from rats submitted to treadmill exercise. Serial stack images obtained with an Olympus Confocal FV-1000 from GFAP immunofluorescence staining in the stratum radiatum of CA1 hippocampal region of rats submitted to treadmill exercise (20 min/day, 5 days/week during four weeks). The images showed an evident reduction in GFAP expression in control/sedentary (SED) rats (a) when compared to exercised (EXE) rats (b). Magnification of 40x, 1.0  m of optical stack thickness, and 15 confocal planes. Scale bars = 50  m.