Improved Method for the Quantification of Motility in Glia and Other Morphologically Complex Cells
Figure 6
Use of paraformaldehyde- (PFA-) fixed cells to reveal nonbiological artifacts that contribute to the motility index scores. (a) The motility of control living cells () was compared with (b) dead cells fixed in paraformaldehyde (). ((c), (d)) Bars indicate the motility values of the two groups normalized to the mean of control values using Method 1 in (c) and Method 2 in (d). Error bars are SEM. (e) Applying a low-pass temporal frequency filter with a threshold of 0.0026 Hz (6.4 min period) reduces the motility index scores (measured using Method 2) of the PFA-fixed cell group by substantially more than the control group, indicating that the filter is effective at eliminating nonbiological noise. Dashed lines indicate full width at half maximum. Scale bars = 10 m.
