System Consolidation of Spatial Memories in Mice: Effects of Enriched Environment

Figure 3

(a) c-Fos protein expression in aCC and IL. aCC: two-way ANOVA post hoc analysis Holm-Sidak method, HC-SC versus HC-EE 𝑃=0.384; statistical significance for EE versus HC-EE at 10, 20, 30, and 50 days, and for SC versus HC-SC at 30 and 50 days, all 𝑃 values < 0.05. Statistical difference between EE and SC at 1 day, 𝑃=0.376; at 10 and 20 days, 𝑃<0.01; at 30 days, 𝑃=0.899; at 50 days, 𝑃=0.383. Statistical difference within EE group were found between 10 and 20 days, and 1 day versus 10, 20, 30, and 50 days, all 𝑃<0.05. Statistical difference within SC group were found between 1 and 30 days, and 10 day versus 30 and 50 days, all 𝑃<0.05. IL: two-way ANOVA post hoc analysis Holm-Sidak method, HC-SC versus HC-EE, 𝑃=0.451; statistical significance for EE versus HC-EE at 10, 20, 30, and 50 days, and for SC versus HC-SC at 30 and 50 days, all 𝑃 values < 0.05. Statistical differences between EE and SC at 1 day, 𝑃=0.300; at 10 and 20 days, 𝑃<0.01; at 30 days, 𝑃=0.084; at 50 days, 𝑃=0.055. Statistical differences within EE group were found between 10 and 20 days, and 1 day versus 10, 20, 30, and 50 days, all 𝑃<0.05. Statistical difference within SC group were found between 1 and 30 days, and 10 day and 30 days, all 𝑃<0.05. ^■Statistical significance between EE and HC-EE; ^□statistical significance between SC and HC-SC; ^∗statistical significance between EE and SC; ^§=statistical significance for factor day within EE group; ^#=statistical significance for factor day within SC group; error bars = s.e.m. (b) Representative panel of c-Fos protein expression in aCC for EE and SC animals, for all recall probe tests; scale bar 100 μm.

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