Combination of Acellular Nerve Graft and Schwann Cells-Like Cells for Rat Sciatic Nerve Regeneration
Figure 4
((a), (b), and (c)) HE staining of transverse nerve slices from each group at 6 w. The regenerated myelinated nerve fibers were sparse; distribution and diameter of the fibers were irregular, but there were no obvious lymphocytic infiltrates. ((d), (e), and (f)) HE staining of transverse nerve slices from each group at 12 w. (d) The number of regenerated myelinated nerve fibers in group A was increased; myelin was relatively dense and irregularly arranged. The number of regenerated myelinated nerve fibers in groups B (e) and C (f) was increased; myelin was dense and regularly arranged; there were blood capillary hyperplasia and no obvious lymphocytic infiltrates. ((g), (h), and (i)) Toluidine blue staining slices from each group at 6 w. (g) The myelin of regenerated myelinated nerve fibers in group A was sparse; the morphology and diameter were irregular. The myelin of regenerated myelinated nerve fibers in groups B (h) and C (i) was dense, but the morphology and diameter were irregular. ((j), (k), and (l)) Toluidine blue staining slices from each group at 12 w. (j) There were large numbers of regenerative and partly myelinated axon in group A and the diameter of them was homogeneous. There were large numbers of regenerative and partly myelinated axon in groups B (k) and C (l) and the diameter of them was homogeneous and myelin sheath was more thicker than group A. ((m), (n), and (o)) Transmission electron microscope from each group at 6 w. (m) The arrangement and thickness of regenerated myelin sheath in group A were irregular. (n) The arrangement of regenerated myelin sheath in group B was more regular than group A, but the thickness of myelin sheath was nonuniform. (o) The arrangement of regenerated myelin sheath in group C was more regular than group A and the thickness of myelin sheath was uniform. ((p), (q), and (r)) Transmission electron microscope from each group at 12 w. (p) The regenerated myelin sheath was thin and irregular, accompanied with Schwann cell proliferation. (q) In group B, the regenerated myelin sheath arranges compactly and thicker, accompanied with Schwann cell proliferation. (r) In group C, the regenerated myelin sheath arranges compactly and thicker than groups A and B, accompanied with Schwann cell proliferation and chondriosome, microtubule, and microfilament inside.
