The Interplay between Reproductive Social Stimuli and Adult Olfactory Bulb Neurogenesis
Figure 2
The accessory olfactory bulb is a site of adult constitutive neurogenesis. (a) Left, schematic representation of a posterior coronal section in the rat OB (cut at the level indicated by the dotted line) showing the anatomical organization of the AOB (green) and the RMS (red) in the core of the OB. Right, schematic representation of a parasagittal section of the rodent forebrain showing the position of the AOB (green) and the SVZ neurogenic niche from which neuroblasts migrate to both the MOB and AOB. (b) Coronal cross-section of the OB showing NeuN labeling in the MOB and AOB-GrL. At higher magnification, examples of BrdU/NeuN colabeled cells (see arrowhead) stained 15 d after the BrdU injection. Scale bars, left 200 μm, middle 50 μm, right 5 μm. (c) 3D reconstruction of EGFP-positive SVZ-derived precursors at 60 days after their homotopic transplantation in a wild type mouse [19]. EGFP-positive cells were found in the GrL and GL AOB layers. All cells show features of mature interneurons with well-developed dendritic arborization and spines, as visible at higher magnification. Scale bars, 100 μm and 10 μm (for higher magnification pictures). AOB: accessory olfactory bulb; MOB: main olfactory bulb; SVZ: subventricular zone; RMS: rostral migratory stream; LV: lateral ventricle; CC: corpus callosum; GrL: granular layer; lot: lateral olfactory tract; epl: external plexiform layer; GL: glomerular layer. ((b) was modified from [12] and (c) was modified from [19]).
