Proliferation in the Alzheimer Hippocampus Is due to Microglia, Not Astroglia, and Occurs at Sites of Amyloid Deposition
Figure 2
Iba1 morphology and proliferation in human hippocampus. (a–c) Representative images for Con, Dem, and AD subjects, respectively, illustrating a similar morphology of Iba1+ (red) cells. (d) Quantitative analysis of Iba+ cell density showed no significant difference between cohorts. Ramified Iba1+ cells throughout the hippocampus coexpress PCNA (arrowheads): (e) representative Aβ and Iba1 distribution, (f) ramified and proliferating Iba1+ cells in the CA3 in the absence of Aβ plaques, (g-h) in the GCL across patient cohorts, and (i-j) near pyramidal neurons expressing APP (black). (k-l) Positive control section from a brain infected with herpes simplex virus encephalitis (HSVE). Infected vessels show activated Iba1+ microglia and coexpression of PCNA (arrowheads). Scale bars (a–c) = 100 μm, (e) = 500 μm, (f) = 50 μm, (g–j) = 20 μm, (k) = 50 μm, and (l) = 20 μm.
