Anandamide, Acting via CB2 Receptors, Alleviates LPS-Induced Neuroinflammation in Rat Primary Microglial Cultures

Figure 2

Release of nitric oxide in cultured rat primary microglia after LPS stimulation and pretreatment with AEA and AM-630, AM-251, or CID-16020046. The results are expressed as the percentage of NO release relative to the nonstimulated control (100%), 24 hours after LPS stimulation. Data are presented as the mean ± SEM and represent the normalized averages derived from 10 to 15 samples per group. Statistical analysis was performed using one-way ANOVA followed by Bonferroni post hoc tests. Statistical values <0.05 were considered significant. * It denotes a significant difference versus the nonstimulated control; #, versus the LPS-stimulated control; $, versus the LPS-stimulated cells treated with AEA.