Research Article
Anandamide, Acting via CB2 Receptors, Alleviates LPS-Induced Neuroinflammation in Rat Primary Microglial Cultures
Figure 4
The results of qPCR analysis of the M1 phenotypic markers Tnf-α, Il1β, Il18, Il6, Cox2, and Nos2 gene expression in rat primary microglial cultures in the presence of the tested compounds. Samples were analyzed 24 hours after LPS stimulation. Data are presented as the mean ± SEM and represent the normalized averages derived from 6 to 8 samples per group. The results are presented as fold-change normalized to the expression of the reference gene Hprt1 and were calculated relative to nonstimulated cells. Statistical analysis was performed using one-way ANOVA followed by Bonferroni post hoc tests; values <0.05 were considered significant. * It denotes a significant difference versus the nonstimulated control; #, versus the LPS-stimulated control.
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