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Neural Plasticity
Volume 2015, Article ID 293423, 10 pages
http://dx.doi.org/10.1155/2015/293423
Research Article

Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats

1Department of Neuroscience and Cell Biology, 301 University Boulevard, University of Texas Medical Branch, TX 77555-1069, USA
2Department of Physiology, College of Korean Medicine, Daegu Haany University, Daegu 706-060, Republic of Korea
3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea
4Department of Oral Physiology, College of Dentistry, Institute of Wonkwang Biomaterial and Implant, Wonkwang University, 344-2 Shinyong Dong, Iksan 570-749, Republic of Korea
5Department of Pain Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA

Received 11 February 2015; Revised 15 April 2015; Accepted 22 April 2015

Academic Editor: Bae Hwan Lee

Copyright © 2015 Hee Young Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Our previous studies suggest that reactive oxygen species (ROS) scavengers have analgesic effect on neuropathic pain through spinal mechanisms in the rat. The studies suggest that superoxide in spinal cord is one of important mediators of persistent pain. To test the hypothesis that increase of superoxide-derived intermediates leads to central sensitization and pain, the effects of an intrathecal injection of chemical ROS donors releasing either , , or H2O2 were examined on pain behaviors. Following treatment with t-BOOH ( donor), dorsal horn neuron responses to mechanical stimuli in normal rats and the changes of neuronal excitability were explored on substantia gelatinosa (SG) neurons using whole-cell patch clamping recordings. Intrathecal administration of t-BOOH or NaOCl ( donor), but not H2O2, significantly decreased mechanical thresholds of hind paws. The responses of wide dynamic range neurons to mechanical stimuli increased after a local application of t-BOOH. The t-BOOH increased the frequency and the amplitude of excitatory postsynaptic potentials, depolarized membrane potential in SG neurons, and increased the frequency of action potentials evoked by depolarizing current pulses. These results suggest that elevated ROS, especially , in the spinal cord sensitized dorsal horn neurons and produced hyperalgesia in normal rats.