Early Maternal Deprivation Enhances Voluntary Alcohol Intake Induced by Exposure to Stressful Events Later in Life

Figure 3

Voluntary alcohol intake following one week of alcohol cessation (a) and following a second week of alcohol cessation combined with restraint stress (b). Data are presented as mean ± S.E.M of the averaged alcohol intake (g/kg/2 h) during the first 2 h of alcohol exposure during the four days period of alcohol exposure (from pnd 57 to pnd 60, panel (a), and from pnd 67 to pnd 70, panel (b)). Rats were exposed to a single episode of early maternal deprivation (24 h on pnd 9, MD) or not deprived (control group, Co) and exposed to voluntary alcohol intake (20% v/v, in a two-bottle choice paradigm) during adolescence and following one week of alcohol cessation and after a second period of alcohol cessation combined with exposure to restraint immobilization stress (30 min. per day under white light conditions) on the last three days of alcohol cessation. Data were analyzed by a two-way ANOVA, ; (A) general effect of sex, (B) general effect of the neonatal condition. -10 cages per experimental group.

(a)

(b)