Early Maternal Deprivation Enhances Voluntary Alcohol Intake Induced by Exposure to Stressful Events Later in Life

Figure 5

Metabolic parameters following stressful events in young adult rats in response to voluntary alcohol intake. ((a), (c)) Growth rate (%) and ((b), (d)) accumulated food intake (g). Rats were exposed to a single episode of early maternal deprivation (24 h on pnd 9, MD) or not deprived (control group, Co) and exposed to voluntary alcohol intake (20% v/v, in a two-bottle choice paradigm) during adolescence and following one week of alcohol cessation (from pnd 57 to pnd 60, upper panels) and after a second period of alcohol cessation combined with exposure to restraint immobilization stress (30 min. per day under white light conditions) on the last three days of alcohol cessation (from pnd 67 to pnd 70, lower panels). Data are presented as mean ± S.E.M and were analyzed by a repeated measures two-way ANOVA (BW gain). Tukey post hoc comparisons: , MD males versus Co males; , Co females versus Co males; or by a two-way ANOVA (accumulated food intake). ; (A) general effect of sex, (B) general effect of the neonatal condition; –20 animals per experimental group.

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