Neural Stem Cell Transplant-Induced Effect on Neurogenesis and Cognition in Alzheimer Tg2576 Mice Is Inhibited by Concomitant Treatment with Amyloid-Lowering or Cholinergic 7 Nicotinic Receptor Drugs

Figure 1

Intrahippocampal hNSCs transplantation affects spatial navigation memory in Tg2576 mice. Learning and memory were assessed in 5- to 7-month-old wild type (wt, ) and Tg2576 (APPswe, ) mice in the pilot Morris water maze task. (a) Escape latency during 4 days of acquisition training. (b) Latency to the former location of the platform or (c) the number of platform crosses in a probe test 24 hours later. compared to wild type. The data are expressed as means ± SEM. (d) The experimental design of the study with 6- to 9-month-old Tg2576 mice () comprised (i) Morris water maze tests with 5 days of acquisition followed 24 hours later by a probe memory test, (ii) daily intraperitoneal injections of 0.3 mg/kg JN403 or 25 mg/kg (+)-phenserine for 5 weeks in total, (iii) bilateral hippocampal injections of ~25,000 hNSCs/hemisphere after 1 week of drug treatment, (iv) repetition of the Morris water maze tasks 5 weeks after transplantation, and (v) sacrifice by transcardial perfusion with PBS. (e) Time taken to find the former platform location in the baseline and follow-up test. (f) Differences in time taken to find the former platform location ( latency; follow-up probe test values minus baseline probe values). (g) Percent time spent in the target quadrant where the platform was located. compared to SHAM + SAL. The data are expressed as means ± SEM.

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