Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis

Figure 1

Efficacy and specificity of the designed siRNAs. (a) Immunoblotting detection of endophilin isoforms and GAPDH in HEK293 cells cotransfected with endophilin-pEGFPC1 plasmids and their corresponding siRNAs or negative control (NC). (b) Effect of each endophilin isoform-specific siRNA on the expression of other endophilin isoforms in HEK293 cells. (c) Neurons cotransfected with GFP (green) and NC or Endo1 siRNA. Cultures were stained using an endophilin1 antibody (red). Scale bar, 20 μm. (d) The percentages of endophilin-positive neurons with each treatment were quantified as the mean ± SEM of three independent experiments. .

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