Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis

Figure 4

Quantitative analysis of sustained neurotransmitter release in endophilin1, endophilin2 (Endo1, 2) double-knockdown neurons. (a) Changes in short-term synaptic depression (STD) in nontransfected neurons evoked by 100 pulses at 5 Hz in neurons cotransfected with Endo1 and Endo2 siRNAs (). (b) Average normalized amplitude of the last 20 responses in Endo1, 2 siRNA-transfected and scrambled negative control- (NC-) transfected neurons; , . (c) Normalized amplitude of excitatory postsynaptic currents (EPSCs) evoked by multiple train stimulation in neurons transfected with control or Endo1, 2 siRNA; . (d) Normalized amplitudes compared among Endo1 siRNA (), Endo2 siRNA (), and Endo1, 2 siRNA () after the 1st, 2nd, 3rd, and 4th train stimulation; .

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