Prophylactic Subacute Administration of Zinc Increases CCL2, CCR2, FGF2, and IGF-1 Expression and Prevents the Long-Term Memory Loss in a Rat Model of Cerebral Hypoxia-Ischemia

Table 1

List of primers to amplify chemokine and growth factors.

Sequence accession in NCBI		Sequence	Position	Amplicon
NM_019305.2	FGF2-f	GGAGTTGTGTCCATCAAGG	736–754	260
	FGF2-r	CCAATGTCTGCTAAGAGCTG	996–977
NM_001082477.2	IGF1-f	TCGTCTTCACATCTCTTCTACC	64–85	381
	IGF1-r	GTGTACTTCCTTTCCTTCTCCT	445–424
NM_031530.1	CCL2-f	GGAGAACTACAAGAGAATCACC	218–239	323
	CCL2-r	GCATCACATTCCAAATCACAC	541–521
NM_021866.1	CCR2-f	CAGGGCTTTATCACATTGGG	388–407	311
	CCR2-r	AGATGACCATGACAAGTAGCG	699–679
AF_10686	GA3PDH-f	AAAACAGTCCATGCCATCAC	360–380	420
	GA3PDH-r	TCCACCACCCTGTTGCTGTA	780–759

The primers were designed using Perl Primer V.1.1.19-1 (graphical design of primer for PCR) and their identity was analyzed using BLAST of the NCBI.