Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B

Figure 2

GFAP and S100B content in the striatum and substantia nigra of rats administered an intrastriatal 6-OHDA injection. Brain samples were analysed at 1, 7, or 21 days after the infusion of 6-OHDA, and the GFAP ((a), (b)) and S100B ((c), (d)) contents were measured by ELISA. Each value represents the mean ± standard error, which was expressed as the percentage of the contralateral unlesioned side (assumed to be 100%, indicated by line) from at least 5 rats per group. ^∗Different from sham-group (two-way ANOVA, ). from sham at 7 days; from sham at 21 days, and different from 6-OHDA at 1 and 7 days (two-way ANOVA followed by Tukey’s post hoc test, ). (e) Immunohistochemistry for GFAP in the striatum at 21 days after surgery. Representative photomicrographs of a 6-OHDA-lesioned rat show the striatum ipsilateral to the 6-OHDA infusion on the right and the striatum contralateral to the infusion on the left. Scale bar = 25 μm.

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