α7 Nicotinic Receptor Promotes the Neuroprotective Functions of Astrocytes against Oxaliplatin Neurotoxicity

Figure 4

TGF-β1 dosage. The concentration of the anti-inflammatory cytokine TGF-β1 was measured in the culture medium of (a) neuron, (b) astrocyte monoculture, and (c) neuron astrocyte coculture. ((a) and (b)) Cells were treated with 1 μM oxaliplatin (48 h) in the absence or presence of 10 μM PNU. (c) Cocultures were treated with increasing concentrations of oxaliplatin (0.3, 1, and 10 μM) in the absence or presence of 10 μM PNU. Results are expressed as pg/mL of TGF-β1 per mg of proteins. Values are expressed as the mean ± S.E.M. of 6 experiments. One-way ANOVA was performed followed by a Bonferroni significant difference procedure. versus control; versus oxaliplatin treatment.

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