Huperzine A Alleviates Mechanical Allodynia but Not Spontaneous Pain via Muscarinic Acetylcholine Receptors in Mice

Figure 3

Application of ambenonium chloride had no effect on spontaneous pain. (a) Ambenonium at 0.01 mg/kg had no effect on the PWTs of the sham and nerve-injury groups (two-way RM ANOVA, sham versus injury: , ; treatments: , , per group). (b) Ambenonium at 0.05 mg/kg increased the PWTs of the nerve-injury group (two-way RM ANOVA, sham versus injury: , ; treatments: , , for sham, for injury; and under SNK test). (c) Increasing the dose of ambenonium to 0.1 mg/kg raised the PWTs in both groups (two-way RM ANOVA, sham versus injury: , ; treatments: , , under Tukey’s test). (d) Time spent in the chambers during the CPP preconditioning period (two-way RM ANOVA, sham versus injury: , ; saline versus drug: , , for sham group, for injury group). (e) Ambenonium at 0.05 mg/kg did not induce preference for the drug-paired chamber in the CPP test (two-way RM ANOVA, sham versus injury: , ; saline versus drug: , , for sham group, for injury group). (f) No difference in the preference time induced by ambenonium at 0.05 mg/kg was detected in the CPP test (t-test, , for sham group, for injury group).

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