Huperzine A Alleviates Mechanical Allodynia but Not Spontaneous Pain via Muscarinic Acetylcholine Receptors in Mice

Figure 5

Infusion of clonidine into the anterior cingulate cortex alleviated spontaneous neuropathic pain. (a) Infusion of clonidine into the ACC increased the PWTs in the sham and nerve-injury groups (two-way RM ANOVA, sham versus injury: , ; treatments: , , for sham, for injury, under Tukey’s test). (b) Infusion of saline into the ACC had no effect on the PWTs (two-way RM ANOVA, sham versus injury: , ; treatments: , , per group). (c) Time spent in the chambers during the preconditioning period in the CPP test (two-way RM ANOVA, sham versus injury: , ; saline versus drug: , , per group). (d) Infusion of clonidine into the ACC induced a preference for the drug-paired chamber in the CPP test (two-way RM ANOVA, sham versus injury: , ; saline versus drug: , , per group, under Tukey’s test). (e) Hup A changed the preference time in the CPP test (t-test, per group ).

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