Chondroitin Sulfate Induces Depression of Synaptic Transmission and Modulation of Neuronal Plasticity in Rat Hippocampal Slices
Effect of CS on paired-pulse facilitation (PPF). To examine if CS might affect presynaptic glutamate release, PPF was measured by calculating the percent facilitation of the second pulse fEPSP over the first pulse (fEPSP2 slope/fEPSP1 slope). (a) The mean PPF was plotted as a function of the interpulse interval and fitted by an exponential function. (b) fEPSPs recorded in response to paired stimuli separated by the indicated interpulse intervals () in control (left) and after 20 min of CS application (300 μM) (right). The fEPSP amplitude evoked corresponds to the 40–50% of maximal response. Data are mean ± SEM of 10 slices. , .