Complex Inflammation mRNA-Related Response in ALS Is Region Dependent
Table 3
Results of mRNA expression assessed by RT-qPCR using GUS-β for normalization.
Frontal cortex
Spinal cord
Control
ALS
value
Control
ALS
value
Proinflammatory signaling
Complement system
C1QTNF7
1.14 ± 0.63
1.42 ± 0.89
1.07 ± 0.43
0.52 ± 0.41
C3AR1
1.23 ± 0.66
1.53 ± 0.94
1.00 ± 0.10
0.94 ± 0.41
Colony stimulating factor
CSF3R
1.16 ± 0.59
1.38 ± 0.69
1.07 ± 0.45
1.37 ± 0.88
Toll-like receptor
TLR4
1.29 ± 0.85
0.93 ± 0.34
1.26 ± 0.91
0.95 ± 0.19
TLR7
1.16 ± 0.59
1.05 ± 0.50
1.08 ± 0.39
2.12 ± 0.90
Cathepsins
CTSC
1.15 ± 0.64
1.62 ± 0.91
1.11 ± 0.52
1.71 ± 0.67
CTSS
1.26 ± 0.83
1.36 ± 0.74
1.05 ± 0.35
2.01 ± 0.93
Interleukins
IL1B
1.32 ± 0.89
3.29 ± 2.70
0.053
1.27 ± 0.81
1.13 ± 1.05
IL6
1.42 ± 1.29
5.11 ± 4.73
1.69 ± 2.19
3.04 ± 2.97
IL6ST
1.04 ± 0.33
1.23 ± 0.48
1.05 ± 0.35
0.84 ± 0.17
IL8
2.19 ± 3.91
1.06 ± 1.16
1.46 ± 1.43
1.41 ± 1.26
TNF family
TNF-α
ND
ND
1.11 ± 0.57
0.95 ± 0.31
TNFRSF1A
1.10 ± 0.52
1.41 ± 0.86
1.04 ± 0.30
0.71 ± 0.11
Anti-inflammatory signaling
IL-10 family
IL10
1.17 ± 0.58
1.40 ± 0.68
1.20 ± 0.70
1.54 ± 0.61
IL10RA
1.13 ± 0.56
1.27 ± 0.75
1.07 ± 0.39
1.55 ± 0.71
0.087
TGF beta family
TGFB1
1.08 ± 0.45
1.19 ± 0.54
1.01 ± 0.15
1.73 ± 0.85
0.063
TGFB2
1.18 ± 0.68
1.41 ± 0.69
1.01 ± 0.17
1.64 ± 0.90
0.07
TLR7, CTSS, and CTSC mRNA are significantly upregulated, and C1QTNF7 and TNFRSF1A mRNA are significantly downregulated in the anterior lumbar spinal cord in ALS; a tendency to increase is also observed for IL10RA, TGFB1, and TGFB2. IL6 is significantly upregulated and IL1B shows a trend to increase in the frontal cortex area 8 in ALS compared to controls. Values are calculated with the ΔΔCT method, using GUS-B as housekeeping gene and control samples as references. ND: not detectable. ; versus controls.
