Localization of Presynaptic Plasticity Mechanisms Enables Functional Independence of Synaptic and Ectopic Transmission in the Cerebellum

Figure 3

Effect of 16 Hz tetanic stimulation on Bergmann glial responses in the presence of presynaptic receptor antagonists. Slices were preincubated with a cocktail of presynaptic receptor antagonists (2 μM DPCPX, 10 μM CGP 52432, 100 μM MPPG, and 5 μM SR141716) for 10 minutes prior to RFS and then for the 30 minutes following RFS. (a) Representative whole-cell recordings of ESCs generated by paired pulse stimulation (100 ms interval) of parallel fibres in transverse cerebellar slices at 0.033 Hz before (first panel), immediately after (second panel), and 30 minutes after (third panel) raised frequency stimulation (RFS, 16 Hz, 15 s). (b) Time course of RFS effect on amplitude of the first pulse in each pair, with RFS delivered at , indicated by the arrow. Data are mean ± s.e.m. from 4 cells. , one-sample -test. (c) Time course of RFS effect on paired pulse ratio after tetanic stimulation at . Data are mean ± s.e.m. from 4 cells.

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