CREB Regulates Experience-Dependent Spine Formation and Enlargement in Mouse Barrel Cortex

Figure 2

Whisker trimming associates with CREB phosphorylation in Contra and Ipsi barrel cortex without perturbing total CREB expression. Representative images of (a) phosphorylated CREB (pCREB, red) and (c) total CREB (CREB, red) expression in immunofluorescence-stained barrel cortex sections. Sections were counterstained with DAPI (blue). Scale bar 30 µm. Histograms showing the average number of (b) pCREB and (d) total CREB positive spots per area from wild type (WT) and mCREB mice in Naïve and trimmed condition (Naïve, Ipsi, and Contra). Values are plotted as number of positive spots per ROI and expressed as mean ± s.e.m. Dotted lines indicate number of spots in barrel cortex of relative Naïve control mice. < 0.05 (difference from relative Naïve controls); < 0.001; < 0.05 (difference between genotypes). : 4 to 6 hemispheres for each group.

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