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Neural Plasticity
Volume 2015, Article ID 651469, 11 pages
Research Article

CREB Regulates Experience-Dependent Spine Formation and Enlargement in Mouse Barrel Cortex

1Laboratory of Psychobiology, Santa Lucia Foundation, 00143 Rome, Italy
2“Tor Vergata” University, 00173 Rome, Italy
3Institute of Cell Biology and Neurobiology (IBCN), National Research Council, 00015 Rome, Italy

Received 11 February 2015; Revised 31 March 2015; Accepted 1 April 2015

Academic Editor: Lucas Pozzo-Miller

Copyright © 2015 Annabella Pignataro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

We confirmed that changes in the number of dendritic spines along apical dendrites of Layer V pyramidal neurons were similar to the ones reported along basal dendrites of the same neurons.

CREB down-regulation does not affect the number of spines in barrel cortex of Naïve mice, as spine density (number of spines per unit length) is comparable between Naïve mCREB and wild type mice along apical dendrites (P>0.05). Trimming condition results in enhanced number of spines in wild type but not mCREB mice (interaction genotype x condition: F2, 60=5.7532, p<0.01). Wild type mice: significant increase of dendritic spine number along apical dendrites of pyramidal neurons in the Contra barrel cortex as compared to Ipsi and Naïve (post-hoc comparisons: Contra vs Naïve p<0.001; Contra vs Ipsi p<0.001). In Ipsi barrel cortex the number of spines was not affected by trimming condition (Ipsi vs Naïve p>0.05). mCREB mice: number of spines was unvaried upon trimming in both Contra and Ipsi as compared to Naïve barrel cortex (p > 0.05 for all comparisons).

No differences between number of dendritic spines along apical dendrites versus basal dendrites were reported (genotype x condition x dendritic category: F2,128=0.30, p>0.05; Post hoc: p>0.05 for all Naïve, Contra and Ipsi apical dendrites vs basal dendrites comparisons in both genotypes).

  1. Supplementary Material