Hunting Increases Phosphorylation of Calcium/Calmodulin-Dependent Protein Kinase Type II in Adult Barn Owls

Figure 4

Experience-dependent regulation of perinuclear pCaMKII. Left panels, frequency histograms of perinuclear pCaMKII signal in (a) Ctrl versus prism juveniles and (b) passive versus active adults. (c) Mean values for all four experimental groups. No difference was observed between controls and prism-wearing juveniles (Mann-Whitney test, ). In contrast, active hunting in adults significantly increased perinuclear pCaMKII intensities ( for Mann-Whitney test). Right panels, frequency histograms of perinuclear pCaMKII/CaMKII signals in (d) Ctrl versus prism and (e) passive versus active. (f) Mean values for all four experimental groups. A small magnitude difference was observed between Ctrl and prism juveniles but was not significant (Mann-Whitney test, ). In contrast, active hunting in adults significantly increased perinuclear pCaMKII/CaMKII signals ( for Mann-Whitney test). All data in this figure is case normalized within experimental group: control juveniles (262 cells), prism-wearing juveniles (392 cells), passive adults (525 cells), and active adults (270 cells).