Limited Effects of an eIF2α^S51A Allele on Neurological Impairments in the 5xFAD Mouse Model of Alzheimer’s Disease

Figure 1

The mutation had no measurable effects on eIF2α phosphorylation and APP processing. Shown are representative immunoblots of (a) p-eIF2α and total eIF2α, (b) human APP and total APP, and (c) α-CTF and β-CTF all prepared from hippocampal homogenates, along with the respective quantitative densitometric data (WT, mice; 5xFAD, mice; 5xFAD;, mice; , mice). (d) Concentrations of abundant Aβ-species, Aβ40 and Aβ42, in 5xFAD hippocampal homogenates were determined using ELISA (5xFAD, mice; 5xFAD;, mice). All data are presented as mean ± SEM. Data were analyzed using two-way ANOVAs with the between-subjects factors 5xFAD genotype/eIF2α genotype (effect of 5xFAD transgenes; effect of eIF2α genotype; 5xFAD eIF2α interaction) and -tests (5xFAD versus 5xFAD;) as appropriate. Statistically significant differences () are denoted by bold font.

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