Generation of New Neurons in Dorsal Root Ganglia in Adult Rats after Peripheral Nerve Crush Injury

Figure 8

After 3 days from crush lesion, cells coexpressing nanog (red) and Ki67 (green) were found (a). A double immunofluorescence performed at day 5 after injury detects sox-2 positive cells (green) surrounding neurons, some of which are peripherin-positive (red) (b, arrows). At the same time point, the same cells are found coexpressing BrdU (green) and nestin (red) (c, arrows). After 7 days from crush lesion, anti-NeuroD is seen to be expressed in many nuclei surrounding the DRG neurons (d). Further characterization of this cell population was done using markers for both immature and mature glial cells. A double labeling using nestin (green) and the immature glial marker GFAP (red) shows that, at day 5 after injury, the two markers colocalize in some cells (e, arrows). However, no colocalization is observed in double labeling of nestin with S-100 (mature glia, in red) (f). Scale bars: a–f = 20 µm.

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