Andrographolide Stimulates Neurogenesis in the Adult Hippocampus

Figure 3

ANDRO increases the density of immature neurons in the dentate gyrus of adult mice. (a) Representative immunostaining of DCX in 2-month-old mice treated with vehicle or ANDRO for 4 weeks. Scale bar: 50 μm. (b) Higher magnification of representative DCX immunostaining in control and ANDRO-treated mice. Scale bar: 30 μm. (c) Representative images of double immunostaining for DCX and NeuN in 10-month-old mice treated with vehicle or ANDRO for 4 weeks. Scale bar: 100 μm. Graph quantification of total number of DCX-positive (DCX+) cells in the GCL 10-month-old mice. Bars represent mean ± S.E. ( mice). , Student’s -test.

(a)

(b)

(c)